BIOLIT 2 line

Femtosecond Fiber Laser for Biophotonics

1050 nm, 70 fs, 2 W, 15 – 40 MHz

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Standout features:

  • Biolit 2 image

    Ultra-short and clean pulses

  • Extremely robust and stable

    Robust and stable

  • Adjustable repetition rate, pulse duration, power

    Flexible repetition rate

  • Maintenance-free & turn-key

    Maintenance-free & turn-key

  • Biolit 2 image

    Integrated dispersion pre-compensation


  • Litilit Multiphoton microscopy

    Multiphoton microscopy

  • Litilit lasers Photopolymerization


  • Ophthalmology


  • Litilit lasers OPO pumping

    OPO pumping

  • Neuroscience


Clean pulses lead to sharp images

The Biolit 2 is a compact, air-cooled femtosecond laser designed for
multiphoton microscopy, biophotonics and other non-linear optics applications.

The industrial-grade device is exceptionally robust, maintenance-free and

A combination of ultra short (typ 55 fs) and clean pulses with
integrated dispersion compensation, excellent beam quality and optimized
repetition rate enables unparalleled quality multiphoton imaging while
preserving the object.


Product data sheet download Product data sheet

Biolit 2
Central wavelength1050 ± 5 nm
Average power> 2 W
Pulse duration< 70 fs (typ. 55 fs)
Pulse duration stability 1) < +/- 3 fs
Pulse strehl ratio> 0.9
Tunable dispersion pre-compensation 2)-8 000 fs² ... +500 fs²
Pulse repetition rate 3)15, 20, 30 or 40 MHz
Analog power control 4)1 - 100%
Beam qualityM² < 1.2 (typ. 1.05)
Beam circularity 5)> 0.9 (typ. 0.94)
Beam diameter (1/e² level)1.5 ± 0.3 mm
Beam divergence (full angle)< 1.5 mrad
Beam pointing (RMS) 1)< 20 μrad
Beam pointing vs temp. < 15 μrad/°C
Pulse Energy Stability (RMS) 6)< 1%
Power Stability (RMS) 1)< 1%
Warm-up time (cold start) < 10 min
Available control interfacesUSB, CAN
Tuneable pulse repetition rate option 7)1 – 20 MHz
Second harmonic option 8) 0.4 W @ 525 nm
Operating voltage24V, 8A (100...240 V AC, 47...63 Hz to 24V AC/DC converter included)
Operating temperature18 – 30 °C
Humiditynon condensing
Transportation/storage temperature- 20 °C – +70 °C
Laser head
Control unit
air (passive)
forced air (fans)
Laser head (L x W x H)
Control unit (L x W x H)
313 x 152 x 107 mm
449 x 370 x 140 mm
Umbilical length3 ± 0.1 m

1) Measured during 8 h operation starting 30 minutes after warm-up. Environmental temperature stability within ± 1 °C.
2) Equivalent of 80 mm of SF10 glass. Even higher dispersion (up to 30’000 fs2) of the external optical system can be pre-compensated in the factory on request. For other pre-compensation options, please contact LITILIT.
3) Factory preset. Other repetition rates are available on request. Please inquire for more details.
4) Attenuation can be controlled in a few different regimes: a) by PC user interface, b) by CAN register, c) by analog input signal (> 5 kHz bandwidth). Beam quality specifications are maintained down to 10% power level.
5) Defined as the worst case beam ellipticity along the z-scan (± 5 × LRayleigh) of the beam.
6) Measured within 10 s time interval.
7) Output repetition rate can be described by formula RR = RR0/ N, where RR0 is fundamental repetition rate and N is integer number. Output power is dependent on both RR and RR0. For power dependence on the repetition rate please contact LITILIT.
8) Biolit-2 SH model also has infrared (1050 nm) output with the same specifications as standard Biolit laser. Both outputs are available simultaneously. Please inquire directly LITILIT for more information.

World patented technology: US10038297, JP6276471, EP3178137, CN106575849.